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Enhancement of PHB Production Process in a Fed-Batch Bioreactor Using Input-Output Linearization Technique with Optimal Setpoints

Sura Srisuddee, Malinee Sriariyanun, Atthasit Tawai, Chanin Panjapornpon, Hesti Wijayanti


Polyhydroxy butyrate (PHB) is a polyester that has been widely applied to formulate bioplastics. A challenge for the PHB production is the enhancement of PHB accumulation in bacterial cells, which can be conducted through the nutrient feeding strategy. A control system based on an input/output (I/O) linearization technique for the PHB production in a fed-batch bioreactor is proposed in this work. The concept of feast/famine phase is employed to provide the optimal desired targets for each time instant through optimization problems. The developed controller is applied to regulate the controlled output to follow the desired setpoints by manipulating the feed flow rate of nutrients. Simulation results show that the proposed control system attempts to follow the optimal desired targets (discrete-time approach) during the process operation. The accumulation of PHB in bacterial cells is around 25% higher than that of the process with a modified PI controller. Control performance tests indicate that the proposed control strategy successfully regulates the process according to the desired targets and handles the process disturbance effectively.


[1] S. Y. Lee, “Bacterial polyhydroxyalkanoates,” Biotechnology and Bioengineering, vol. 49, no. 1, pp. 1–14, 1996.

[2] M. Majone, P. Massanisso, A. Carucci, K. Lindrea, and V. J. W. S. Tandoi, “Influence of storage on kinetic selection to control aerobic filamentous bulking,” Water Science and Technology, vol. 34, no. 5–6, pp. 223–232, 1996.

[3] S. Ochoa, C. García, and W. Alcaraz, “Real‐time optimization and control for polyhydroxybutyrate fed‐batch production at pilot plant scale,” Journal of Chemical Technology and Biotechnology, vol. 95, no. 12, pp. 3221–3231, 2020.

[4] P. Cougnon, D. Dochain, M. Guay, and M. Perrier, “On-line optimization of fedbatch bioreactors by adaptive extremum seeking control,” Journal of Process Control, vol. 21, no. 10, pp. 1526–1532, 2011.

[5] M. Titica, D. Dochain, and M. Guay, “Adaptive extremum seeking control of fed-batch bioreactors,” European Journal of Control, vol. 9, no. 6, pp. 618– 631, 2003.

[6] W. Wu, S.-Y. Lai, M.-F. Jang, and Y.-S. Chou, “Optimal adaptive control schemes for PHB production in fed-batch fermentation of Ralstonia eutropha,” Journal of Process Control, vol. 23, no. 8, pp. 1159–1168, 2013.

[7] I. Cornet, D. Dochain, B. Ramsay, J. Ramsay, and M. Perrier, “Application of an adaptive linearizing inferential controller to a PHB process,” Biotechnology and Biotechnological Equipment, vol. 9, no. 1, pp. 96–102, 1995.

[8] W. Chonwattana, C. Panjapornpon, A. Tawai, and T. J. C. Dechakupt, “Model-based estimation and control of interface level in a two-phase vertical decanter: A case study of palm-oil/water system,” Computers and Chemical Engineering, vol. 108, pp. 372–381, 2018.

[9] A. Tawai and C. Panjapornpon, “Input–output linearizing control strategy for an ethylene dichloride cracking furnace using a coupled PDE-ODE model,” Industrial and Engineering Chemistry Research, vol. 55, no. 3, pp. 683–691, 2016.

[10] D. J. L. Faccin, I. Martins, N. S. M. Cardozo, R. Rech, M. A. Z. Ayub, T. Lívio M. Alves, R. Gambetta, and A. R. Secchi, “Optimization of C : N ratio and minimal initial carbon source for poly (3‐hydroxybutyrate) production by Bacillus megaterium,” Journal of Chemical Technology and Biotechnology, vol. 84, no. 12, pp. 1756– 1761, 2009.

[11] P. R. Patnaik, “Dispersion optimization to enhance PHB production in fed-batch cultures of Ralstonia eutropha,” Bioresource Technology, vol. 97, no. 16, pp. 1994–2001, 2006.

[12] J. M. L. Dias , P. C. Lemos, L. S. Serafim, C. Oliveira, M. Eiroa, M. G. E. Albuquerque, A. M. Ramos, R. Oliveira, and M. A. M. Reis, “Recent advances in polyhydroxyalkanoate production by mixed aerobic cultures: From the substrate to the final product,” Macromolecular Bioscience, vol. 6, no. 11, pp. 885–906, 2006.

[13] S. Shahhosseini, “Simulation and optimisation of PHB production in fed-batch culture of Ralstonia eutropha,” Process Biochemistry, vol. 39, no. 8, pp. 963–969, 2004.

[14] C. García, W. Alcaraz, A. Acosta-Cárdenas, and S. J. B. Ochoa, “Application of process system engineering tools to the fed-batch production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from a vinasses–molasses Mixture,” Bioprocess and Biosystems Engineering, vol. 42, no. 6, pp. 1023– 1037, 2019.

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DOI: 10.14416/j.asep.2021.02.003


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