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Optimization of Parameters for the Extraction of Phenolic Antioxidants from Boxberry Tree (Myrica Esculenta) Bark Using Response Surface Methodology

Do Thi Kieu Trinh, Nguyen Thi Thanh Tinh, Ho Thi Thu Hoa, Nguyen Tien An, Pham Ngoc Tuan, Phan Hoang Dai

Abstract


The boxberry tree (Myrica esculenta) bark has been known to have multiple health benefits and is used as a traditional medicine. A critical gap in knowledge exists on a simple but effective method to isolate the bioactive components from the bark. This study aimed to optimize the operating conditions, including temperature, ethanol concentration, and time, for the extraction of phenolic antioxidants from the boxberry bark sample using a response surface methodology. Results showed that the second-order polynomial regression models were statistically significant and sufficient to estimate the responses. Response surface optimization for all responses was successfully carried out to determine the optimum extraction conditions, which were a temperature, an ethanol concentration, and an extraction time of 75.8 °C, 48.3% (v/v), and 117 min, respectively. At these conditions, total phenolic and total flavonoid contents, 3-ethylbenzothiazoline-6-sulphonic acid diammonium salt (ABTS) scavenging capacity, and ferric-reducing antioxidant power were predicted to be 205.9 mg GAE/100 g, 37.8 mg CE/100 g, 271.3 mg AAE/100 g, and 111.4 mg AAE/100 g, respectively. The insignificant difference between the estimated and the experimental values suggested that the predictive models were valid to predict the process outcomes.

Keywords



[1] A. Kabra, R. Sharma, S. Singla, R. Kabra, and U. S. Baghel, “Pharmacognostic characterization of Myrica esculenta leaves,” Journal of Ayurveda and Integrative Medicine, vol. 10, no. 1, pp. 18–24, 2019, doi: 10.1016/j.jaim.2017.07.012.

 

[2] Y. Khan, H. Sagrawat, N. Upmanyu, and S. Siddique, “Anxiolytic properties of Myrica nagi bark extract,” Pharmaceutical Biology, vol. 46, no. 10–11, pp. 757–761, 2008, doi: 10.1080/13880200802315436.

 

[3] V. K. Jain and B. Jain, “Antihelminthic activity of ethanolic extract of bark of Myrica esculenta,” International Journal of Pharmaceutical Sciences and Research, vol. 1, no. 11, pp. 129–131, 2010.

 

[4] K. G. Patel, N. J. Rao, V. G. Gajera, P. A. Bhatt, K. V. Patel, and T. R. Gandhi, “Anti-allergic activity of stem bark of Myrica esculenta Buch.-Ham. (Myricaceae),” Journal of Young Pharmacists, vol. 2, no. 1, pp. 74–78, 2010, doi: 10.4103/0975-1483.62219.

 

[5] D. Kumar, Z. A. Bhat, P. Singh, S. S. Bhujbal, and R. S. Deoda, “Antihistaminic activity of aqueous extract of stem bark of Ailanthus excelsa Roxb.,” Pharmacognosy Research, vol. 3, no. 3, pp. 220–224, 2011, doi: 10.4103/0974-8490.85014.

 

[6] T. Patel, C. Rajshekar, and R. Parmar, “Mast cell stabilizing activity of Myrica nagi bark,” Journal of Pharmacognosy and Phytotherapy, vol. 3, no. 8, pp. 114–117, 2011.

 

[7] A. Kabra, N. Martins, R. Sharma, R. Kabra, and U. S. Baghel, “Myrica esculenta Buch.-Ham. ex D. Don: A natural source for health promotion and disease preventdion,” Plants, vol. 8, no. 6, pp. 1–21, 2019, doi: 10.3390/plants8060149.

 

[8] T. K. Dua, S. Joardar, P. Chakraborty, S. Bhowmick, A. Saha, V. De Feo, and S. Dewanjee, “Myricitrin, a glycosyloxyflavone in Myrica esculenta bark ameliorates diabetic nephropathy via improving glycemic status, reducing oxidative stress, and suppressing inflammation,” Molecules, vol. 26, no. 2, Jan. 2021, doi: 10.3390/molecules 26020258.

 

[9] B. Sapkota, A. Acharya, B. Dangi, and A. Hv, “Evaluation of antipsychotic activity of ethanolic bark extract of Myrica esculenta in rats,” Pharmaceutical Sciences and Research (PSR), vol. 7, no. 3, pp. 153–158, 2020, .

 

[10] P. Sood and R. Shri, “A review on ethnomedicinal, phytochemical and pharmacological aspects of Myrica esculenta,” Indian Journal of Pharmaceutical Sciences, vol. 80, no. 1, pp. 2–13, 2018.

 

[11] N. Singh, S. Khatoon, N. Srivastava, A. K. Singh Rawat, and S. Mehrotra, “Qualitative and quantitative standardization of Myrica esculenta Buch.-Ham. stem bark by use of HPTLC,” Journal of Planar Chromatography - Modern TLC, vol. 22, no. 4, pp. 287–291, 2009, doi: 10.1556/JPC.22.2009.4.9.

 

[12] B. Srivastava, V. C. Sharma, P. Pant, N. K. Pandey, and A. D. Jadhav, “Evaluation for substitution of stem bark with small branches of Myrica esculenta for medicinal use – A comparative phytochemical study,” Journal of Ayurveda and Integrative Medicine, vol. 7, no. 4, pp. 218–223, 2016, doi: 10.1016/j.jaim.2016.08.004.

 

[13] N. Chairerk, P. Pongyeela, J. Chungsiriporn, and N. Rakmak, “Ethanol extraction of active ingredients and antioxidants from germinated sangyod rice,” Applied Science and Engineering Progress, vol. 14, no. 1, pp. 52–59, 2021, doi: 10.14416/j.asep.2019.03.003.

 

[14] D. Bas and I. H. Boyaci, “Modeling and optimization I: Usability of response surface methodology,” Journal of Food Engineering, vol. 78, no. 3, pp. 836–845, 2007.

 

[15] M. A. Bezerra, R. E. Santelli, E. P. Oliveira, L. S. Villar, and L. A. Escaleira, “Response surface methodology (RSM) as a tool for optimization in analytical chemistry,” Talanta, vol. 76, no. 5, pp. 965–977, 2008, doi: 10.1016/ j.talanta.2008.05.019.

 

[16] U. T. N. Ho, L. T. M. Tran, A. Q. Dinh, and A. T. Nguyen, “Response surface optimization of ethanolic extraction of antioxidants from artichoke leaves,” Journal of Food Processing and Preservation, vol. 39, no. 6, pp. 1036–1044, 2015, doi: 10.1111/jfpp.12318.

 

[17] M. Pinelo, M. Rubilar, J. Sineiro, and M. J. Núñez, “Extraction of antioxidant phenolics from almond hulls (Prunus amygdalus) and pine sawdust (Pinus pinaster),” Food Chemistry, vol. 85, no. 2, pp. 267–273, 2004, doi: 10.1016/ j.foodchem.2003.06.020.

 

[18] J. Chandrasekhar, M. C. Madhusudhan, and K. S. M. S. Raghavarao, “Extraction of anthocyanins from red cabbage and purification using adsorption,” Food and Bioproducts Processing, vol. 90, no. 4, pp. 615–623, 2012, doi: 10.1016/j.fbp.2012.07.004.

 

[19] B. Singh, H. K. Sharma, and B. C. Sarkar, “Optimization of extraction of antioxidants from wheat bran (Triticum spp.) using response surface methodology,” Journal of Food Science and Technology, vol. 49, no. 3, pp. 294–308, 2011, doi: 10.1007/s13197-011-0276-5.

 

[20] M. C. Macawile and J. Auresenia, “Utilization of supercritical carbon dioxide and Co-solvent n-hexane to optimize oil extraction from Gliricidia sepium seeds for biodiesel production,” Applied Science and Engineering Progress, vol. 15, no. 1, 2022, doi: 10.14416/j.asep.2021.09.003.

 

[21] V. L. Singleton and J. J. A. Rossi, “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents,” American Journal of Enology and Viticulture, vol. 16, no. 3, pp. 144–158, 1965, doi: 10.12691/ijebb-2-1-5.

 

[22] K. K. Adom and R. H. Liu, “Antioxidant activity of grains,” Journal of Agricultural and Food Chemistry, vol. 50, no. 21, pp. 6182–6187, 2002, doi: 10.1021/jf0205099.

 

[23] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology and Medicine, vol. 26, pp. 1231–1237, 1999.

 

[24] S. I. Mussatto, L. F. Ballesteros, S. Martins, and J. a. Teixeira, “Extraction of antioxidant phenolic compounds from spent coffee grounds,” Separation and Purification Technology, vol. 83, pp. 173–179, 2011, doi: 10.1016/j.seppur. 2011.09.036.

 

[25] A. Mokrani and K. Madani, “Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit,” Separation and Purification Technology, vol. 162, pp. 68–76, 2016, doi: 10.1016/j.seppur.2016.01.043.

 

[26] M. Irakli, P. Chatzopoulou, and L. Ekateriniadou, “Optimization of ultrasound-assisted extraction of phenolic compounds: Oleuropein, phenolic acids, phenolic alcohols and flavonoids from olive leaves and evaluation of its antioxidant activities,” Industrial Crops and Products, vol. 124, pp. 382–388, 2018, doi: 10.1016/j.indcrop. 2018.07.070.

 

[27] S. Nipornram, W. Tochampa, P. Rattanatraiwong, and R. Singanusong, “Optimization of low power ultrasound-assisted extraction of phenolic compounds from mandarin (Citrus reticulata Blanco cv. Sainampueng) peel,” Food Chemistry, vol. 241, pp. 338–345, 2018, doi: 10.1016/ j.foodchem.2017.08.114.

 

[28] C.-Y. Gan and A. A. Latiff, “Optimisation of the solvent extraction of bioactive compounds from Parkia speciosa pod using response surface methodology,” Food Chemistry, vol. 124, no. 3, pp. 1277–1283, 2011, doi: 10.1016/j.foodchem. 2010.07.074.

 

[29] J. Saha, A. Biswas, A. Chhetri, and P. K. Sarkar, “Response surface optimisation of antioxidant extraction from kinema, a Bacillus-fermented soybean food,” Food Chemistry, vol. 129, no. 2, pp. 507–513, 2011, doi: 10.1016/j.foodchem. 2011.04.108.

 

[30] D. Sun, Z. Zhao, H. Wong, and L. Y. Foo, “Tannins and other phenolics from Myrica esculenta bark,” Phytochemistry, vol. 27, no. 2, pp. 579–583, 1988.

 

[31] K. G. Patel, V. G. Patel, K. V. Patel, and T. R. Gandhi, “Validated HPTLC method for quanti­fication of myricetin in the stem bark of Myrica esculenta Buch. Ham. Ex D. Don, myricaceae,” Journal of Planar Chromatography - Modern TLC, vol. 23, no. 5, pp. 326–331, 2010, doi: 10.1556/JPC.23.2010.5.4.

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DOI: 10.14416/j.asep.2023.09.001

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