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Effect of Pepsin and Hydrolysis Time on Antioxidative Activity of CollagenHydrolysate from Chicken Feet through Response Surface Methodology

Mattayanee Lomjabok, Nisanarth Krasaechol, Samart Sai-Ut


เท้าไก่ใช้เป็นแหล่งของคอลลาเจนและเจลาตินที่มีคุณภาพสูง ซึ่งนำมาผลิตเป็นโปรตีนไฮโดรไลเสตที่สามารถออกฤทธิ์ทางชีวภาพเป็นการทำให้ได้ผลิตภัณฑ์ที่มีมูลค่าเพิ่มมากขึ้น โดยการวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาผลของปริมาณเปปซิน(0.02–5% w/w) ร่วมกับระยะเวลาในการย่อย (2–8 ชั่วโมง) เพื่อผลิตคอลลาเจนไฮโดรไลเสตจากเท้าไก่ต่อความสามารถในการยับยั้งการเกิดออกซิเดชันด้วยวิธีพื้นที่ผิวตอบสนอง (Response Surface Methodology) ซึ่งออกแบบการทดลองแบบ Central Composite Design (CCD) จากผลการวิจัยพบว่า ปริมาณเปปซินร่วมกับระยะเวลาที่ใช้ในการย่อยมีความสัมพันธ์กับปริมาณโปรตีนและปริมาณโปรตีนที่ไม่ชอบน้ำโดยจะเพิ่มมากขึ้นเมื่อเพิ่มปริมาณเปปซิน อีกทั้งยังสัมพันธ์กับความสามารถในการต้านอนุมูลอิสระด้วยวิธี ABTS ที่จะเพิ่มมากขึ้นในระดับหนึ่งและลดลงเมื่อปริมาณเปปซินและระยะเวลาในการย่อยเพิ่มขึ้น และเมื่อนำมาทวนสอบความแม่นยำของสมการจะเห็นได้ว่าทั้ง 3 ค่าตอบสนองมี Error (%) ต่ำ เนื่องจากค่าตอบสนองที่ได้จากการทดลองมีค่าใกล้เคียงกับการทำนาย โดยสภาวะที่เหมาะสมที่สุดในการผลิตคอลลาเจนไฮโดรไลเสตจากเท้าไก่ให้มีความสามารถในการต้านอนุมูลอิสระด้วยวิธี ABTS มากที่สุด คือการใช้เปปซิน 2.08% (w/w) ร่วมกับใช้ระยะเวลาในการย่อย 4.48 ชั่วโมง

Chicken feet contain high quality collagen and gelatin, which can produce proteins hydrolysate with bioactivity, resulting in higher value-added products. The objective of this study was to study the effect of pepsin content (0.02–5% w/w) in combination with digestion time (2–8 hours) to produce collagen hydrolysate from chicken feet with antioxidative activities through Response Surface Methodology (RSM). The experiment was Central Composite Design (CCD). The results showed that pepsin concentration and digestion time were related to the protein content and the hydrophobicity protein content. Increasing in pepsin concentration made protein content and hydrophobicity value of collagen hydrolysate higher. In addition, ABTS radical scavenging activity increased up to a certain level, and then, decreased when the pepsin concentration and digestion time increased more. To confirm the validity of the statistical model, all responses had low error value (%) because the observation values were close to the predicted values. Optimization by RSM showed that using 2.08% (w/w) pepsin with the digestion time of 4.48 hours could produce collagen hydrolysate with the highest ABTS radical scavenger activity.


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DOI: 10.14416/j.kmutnb.2020.12.005

ISSN: 2985-2145