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Influence of Acetic Acid Pretreatment and its Residue on Bioethanol and Biogas Production from Water Hyacinth

Diana Jose, Atthasit Tawai, Divya Divakaran, Malinee Sriariyanun, Vanarat Phakeenuya, Yu-Shen Cheng, Prapakorn Tantayotai

Abstract


Water hyacinth, an invasive species in natural water habitats, poses ecological challenges but also holds promise as a biofuel resource due to its abundant biomass. To optimize sugar yield for biofuel production, this study focuses on pretreating water hyacinth with acetic acid (AC) using Response Surface Methodology (RSM). Comparing AC, hydrochloric acid (HA), and untreated samples, AC-pretreated samples yielded the highest sugar content at 28.26 g/100 g of biomass, nearly 1.97 times higher than that of untreated samples. Additionally, AC-pretreated samples produced the maximum biogas (2573 mL) after 45 days of anaerobic digestion, while HA pretreatment yielded the highest ethanol production (9.32 g/L) within 48 h. The structural changes in the pretreated and untreated water hyacinth samples were compared using FTIR analysis, and the results showed that the pretreatment approaches exposed more cellulose to hydrolysis. Furthermore, the study investigated the impact of post-washing following acid pretreatment of water hyacinth and discovered that AC residues had no adverse effects, suggesting that the post-washing phase was unnecessary for ethanol production. These findings demonstrate that AC pretreatment can effectively enhance hydrolysis and biofuel production and that eliminating post-washing may reduce wastewater generated during the pretreatment process.


Keywords



[1] I. N. Budiyono, S. Widiasa, and S. Johari, “The kinetic of biogas production rate from cattle manure in batch mode,” International Journal of Chemical and Biomolecular Engineering, vol. 3, pp. 39–44, 2010.

 

[2] T. Phusantisampan, N. Kitiborwornkul, “Progress in chemical pretreatment of lignocellulose biomass for applications in biorefinery” The Journal of King Mongkut's University of Technology North Bangkok, vol. 32, no. 4, pp. 1087–1101, 2022, doi: 10.14416/j.kmutnb. 2022.09.018.

 

[3] A. Ganguly, P. K. Chatterjeea, and A. Dey, “Studies on ethanol production from water hyacinth – A review,” Renewable and Sustainable Energy Reviews, vol. 16, no. 1, pp. 966–972, 2012.

 

[4] C. P. Craft, S. Megonigal, J. Broome, R. Stevenson, J. Freese, L. Cornell, S. Zheng, and J. Sacco, “The pace of ecosystem development of constructed Spartina alterniflora marshes,” Ecological Applications, vol. 13, no. 5, pp. 1417–1432, 2003.

 

[5] G. Aweke, “The water hyacinth (Eichhornia crassipes) in Ethiopia,” Bulletin des Séances, Académie Royale des Sciences d'Outre-Mer, vol. 39, no. 3, pp. 399–404, 1993.

 

[6] C. C. Gunnarsson and C. M. Petersen, “Water hyacinths as a resource in agriculture and energy production: A literature review,” Waste Management, vol. 27, no. 1, pp. 117–129, 2007.

 

[7] K. Poddar, L. Mandal, and G. C. Banerjee, “Studies on water hyacinth (Eichhornia crassipes)— chemical composition of the plant and water from different habitats,” Indian Veterinary Journal, vol. 68, pp. 833–837, 1991.

 

[8] J. Gressel, “Transgenics are imperative for biofuel crops,” Plant Science, vol. 174, no. 3, pp. 246–263, 2008.

 

[9] I. Sunwoo, J. E. Kwon, T. H. Nguyen, and S. K. Kim, “Ethanol production from water hyacinth (Eichhornia crassipes) hydrolysate by hyper-thermal acid hydrolysis, enzymatic saccharification and yeasts adapted to high concentration of xylose,” Bioprocess and Biosystems Engineering, vol. 42, no. 8, pp. 1367– 1374, 2019.

 

[10] J. L. Varanasi, and D. Das, “Maximizing biohydrogen production from water hyacinth by coupling dark fermentation and electrohydrogenesis,” International Journal of Hydrogen Energy, vol. 45, no. 8, pp. 5227–5238, 2020.

 

[11] S. S. Ali, T. Elsamahy, A. Abdelfattah, A. M. Mustafa, M. A. Khalil, S. G. Mastropetros, M. Kornaros, J. Sun, and M. Azab, “Exploring the potential of anaerobic co-digestion of water hyacinth and cattle dung for enhanced biomethanation and techno-economic feasibility,” Fuel, vol. 329, no. 1, 2022, Art. no. 125397.

 

[12] Y. S. Cheng, P. Mutrakulcharoen, S. Chuetor, K. Cheenkachorn, P. Tantayotai, E. J. Panakkal, and M. Sriariyanun, “Recent situation and progress in biorefining process of lignocellulosic biomass: toward green economy,” Journal of Applied Science and Engineering, vol. 13, no. 4, pp. 299–311, 2020.

 

[13] E. J. Panakkal, J. Ratanapoompinyo, N. Kitiborwornkul, P. Yasurin, S. Asavasanti, W. Rodiahwati, P. Tantayotai, and M. Sriariyanun, “Production of food flavouring agents by enzymatic reaction and microbial fermentation,” Applied Science and Engineering Progress, vol. 14, no. 3, pp. 297– 312, 2021.

 

[14] M. Sriariyanun, M. P. Gundupalli, V. Phakeenuya, T. Phusantisampan, Y. S. Cheng, P. Venkatachalam, “Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms,” Journal of Applied Science and Engineering, vol. 27, no. 2, pp. 1985– 2005, 2024.

 

[15] D. Jose, A. Tawai, D. Divakaran, D. Bhattacharyya, P. Venkatachalam, P. Tantayotai, and M. Sriariyanun, “Integration of deep eutectic solvent in biorefining process of lignocellulosic biomass valorization,” Bioresource Technology Reports, vol. 21, pp. 101365, 2023.

 

[16] S. Sarto, R. Hildayati, and I. Syaichurrozi, “Effect of chemical pretreatment using sulfuric acid on biogas production from water hyacinth and kinetics,” Renewable Energy, vol. 132, pp. 335– 350, 2019.

 

[17] M. P. Gundupalli, S. T. A. Sahithi, Y. S. Cheng, P. Tantayotai, and M. Sriariyanun, “Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass,” Bioprocess and Biosystems Engineering, vol. 44, no. 11, pp. 2331– 2344, 2021.

 

[18] P. Tantayotai, M. P. Gundupalli, E. J. Panakkal, M. Sriariyanun, K. Rattanaporn, and D. Bhattacharyya, “Differential influence of imidazolium ionic liquid on cellulase kinetics in saccharification of cellulose and lignocellulosic biomass substrate,” Applied Science and Engineering Progress, vol. 15, no. 3, 2022, Art. no. 5510, doi: 10.14416/j. asep.2021.11.003.

 

[19] B. Dharmalingam, P. Tantayotai, E. J. Panakkal, K. Cheenkachorn, S. Kirdponpattara, M. P. Gundupalli, Y. S. Cheng, and M. Sriariyanun, “Organic acid pretreatments and optimization techniques for mixed vegetable waste biomass conversion into biofuel production,” BioEnergy Research, vol. 16, pp. 1667–1682, 2023.

 

[20] K. Rattanaporn, P. Tantayotai, T. Phusantisampan, P. Pornwongthong, and M. Sriariyanun, “Organic acid pretreatment of oil palm trunk: Effect on enzymatic saccharification and ethanol production,” Bioprocess and Biosystem Engineering, vol. 41, no. 4, pp. 467–477, 2018.

 

[21] G. Licitra, T. Hernández, and P. V. Soest, “Use of detergents in the analysis of fibrous feeds. IV: Determination of plant cell-wall constituents,” Journal of Association of Official Analytical Chemists, vol. 50, pp. 50–55, 1967, doi: 10.1093/ jaoac/50.1.50.

 

[22] APHA, Standard Methods for the Examination of Water and Wastewater, 21st ed., Washington DC: Public Health Association/American Water Works Association/Water Environment Federation, 2005.

 

[23] M. P. Gundupalli, P. Tantayotai, S. Chuetor, K. Cheenkachorn, S. Joshi, D. Bhattacharyya, and M. Sriariyanun, “Improvement of water hyacinth bioconversion by different organic and mineral acid pretreatment and the effect of post pretreatment washing,” BioEnergy Research, vol. 16, pp. 1718–1732, 2023.

 

[24] T. K. Ghose, “Measurement of cellulase activities,” Pure and Applied Chemistry, vol. 59, no. 2, pp. 257–268, 1987.

 

[25] P. Tantayotai, K. Rattanaporn, S. Tepaamorndech, K. Cheenkachorn, and M. Sriariyanun, “Analysis of an ionic liquid and salt tolerant microbial consortium which is useful for enhancement of enzymatic hydrolysis and biogas production,” Waste and Biomass Valorization, vol. 10, no. 6, pp. 1481–1491, 2019.

 

[26] G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959.

 

[27] K. Rattanaporn, P. Tantayotai, T. Phusantisampan, P. Pornwongthong, and M. Sriariyanun, “Organic acid pretreatment of oil palm trunk: Effect on enzymatic saccharification and ethanol production,” Bioprocess and Biosystems Engineering, vol. 41, no. 4, pp. 467–477, 2018.

 

[28] S. Chuetor, E. J. Panakkal, T. Ruensodsai, K. Cheenkachorn, S. Kirdponpattara, Y. S. Cheng, and M. Sriariyanun, “Improvement of enzymatic saccharification and ethanol production from rice straw by recycled ionic liquid: Effect of anti-solvent mixture,” Bioengineering, vol. 9, no. 3, p. 115, 2022.

 

[29] T. A. Lloyd, and C. E. Wyman, “Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids,” Bioresource Technology, vol. 96, no. 18, pp. 1967–1977, 2005.

 

[30] M. P. Gundupalli, A. S. T. Sahithi, Y. S. Cheng, P. Tantayotai, and M. Sriariyanun, “Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass,” Bioprocess and Biosystems Engineering, vol. 44, no. 11, pp. 2331–2344, 2021.

 

[31] S. Areeya, E. J. Panakkal, M. Sriariyanun, T. Kangsadan, A. Tawai, S. Amornraksa, U. W. Hartley, and P. Yasurin, “A review on chemical pretreatment of lignocellulosic biomass for the production of bioproducts: Mechanisms, challenges and applications,” Applied Science and Engineering Progress, vol. 16, no. 3, 2023, Art. no. 6767, doi: 10.14416/j.asep.2022.08.001.

 

[32] H. Kangas, T. Liitiä, S. Rovio, T. Ohra-Aho, H. Heikkinen, T. Tamminen, and K. Poppius-Levlin, “Characterization of dissolved lignins from acetic acid Lignofibre (LGF) organosolv pulping and discussion of its delignification mechanisms,” Holzforschung, vol. 69, pp. 247–256, 2015.

 

[33] K. B. Dharmalingam, P. Tantayotai, and R. B. N. Prasad, “Organic acid pretreatment and optimization technique for mixed vegetable waste biomass: effects on biogas production and kinetic modelling,” Bioenergy Research, vol. 15, no. 2, pp. 773–783, 2022.

 

[34] G. Wan, Q. Zhang, M. Li, Z. Jia, C. Guo, B. Luo, S. Wang, and D. Min, “How pseudo-lignin is generated during dilute sulfuric acid pretreatment,” Journal of Agricultural and Food Chemistry, vol. 67, no. 36, pp. 10116–10125, 2019.

 

[35] D. Haldar, P. Dey, A. K. Patel, C. D. Dong, and R. R. Singhania, “A critical review on the effect of lignin redeposition on biomass in controlling the process of enzymatic hydrolysis,” Bioenergy Research, vol. 15, pp. 863–874, 2022.

 

[36] E. P. Dagninoa, E. R. Chamorroa, S. D. Romano, F. E. Felissia, and M. C. Area, “Optimization of the acid pretreatment of rice hulls to obtain fermentable sugars for bioethanol production,” Industrial Crops and Products, vol. 42, pp. 363– 368, 2013.

 

[37] Q. Ji, C. P. Tan, A. E. G. A. Yagoub, L. Chen, D. Yan, and C. Zhou, “Effects of acidic deep eutectic solvent pretreatment on sugarcane bagasse for efficient 5-hydroxymethylfurfural production,” Energy Technology, vol. 9, no. 9, 2021, Art. no. 2100396, doi: 10.1002/ente.202100396.

 

[38] T. S. Marimuthu and R. Atmakuru, “Isolation and characterization of cellulose nanofibers from the aquatic weed water hyacinth—Eichhornia crassipes,” Carbohydrate Polymers, vol. 87, no. 2, pp. 1701–1705, 2012.

 

[39] K. M. Lee, J. Y. Hong, and W. Y. Tey, “Combination of ultrasonication and deep eutectic solvent in the pretreatment of lignocellulosic biomass for enhanced enzymatic saccharification,” Cellulose, vol. 28, pp. 1513–1526, 2021.

 

[40] Z. Guo, Q. Zhang, T. You, X. Zhang, F. Xu, and Y. Wu, “Short-time deep eutectic solvent pretreatment for enhanced enzymatic saccharification and lignin valorization,” Green Chemistry, vol. 21, p. 3099, 2019.

 

[41] G. Suresh, H. Kopperi, and S. V. Mohan, “Hydrothermal processing of agar waste to levulinic acid and fermentation of hydrolysate to bioethanol,” Bioresource Technology, vol. 382, 2023, Art. no. 129063.

 

[42] D. Greetham, A. J. Hart, and G. A. Tucker, “Presence of low concentrations of acetic acid improves yeast tolerance to hydroxymethylfurfural (HMF) and furfural,” Biomass and Bioenergy, vol. 85, pp. 53–60, 2016.

 

[43] M. Zeng, S. Zhao, S. Yang, S. Ding, Y. Huang, L. Lin, and H. Huang, “Pretreatment optimization of corn stover using wet disk milling and the enzymatic hydrolysis of the resulting milling fractions,” Bioresource Technology, vol. 216, pp. 382–387, 2016.

 

[44] X. Li, T. H. Kim, N. P. Nghiem, and Y. Y. Lee, “Comparison of different washing techniques for effective removal of inhibitors from lignocellulosic hydrolysates,” Biotechnology for Biofuels, vol. 7, no. 1, pp. 1–14, 2014.

 

[45] P. Yu, X. Chen, J. Jiang, Y. Chen, Z. Wang, X. Wang, and P. Ouyang, “Impact of lignocellulosic biomass washing pretreatment on Saccharomyces cerevisiae fermentation,” Bioresource Technology, vol. 268, pp. 337–344, 2018.

 

[46] H. B. Klinke, A. B. Thomsen, and B. K. Ahring, “Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass,” Applied Microbiology and Biotechnology, vol. 66, no. 1, pp. 10–26, 2004.

 

[47] K. Liu, X. Wang, L. Han, J. Zhang, and Z. Liu, “Evaluation of different pretreatment methods on the growth of Saccharomyces cerevisiae for bioethanol production from corn stover,” Bioresource Technology, vol. 241, pp. 112–119, 2017.

 

[48] A. K. Pandey, A. Sharma, K. K. Jain, and S. Jain, “Comparative evaluation of different pre-treatments on biogas production from water hyacinth biomass,” Renewable Energy, vol. 166, pp. 155–164, 2021.

 

[49] V. R. Nimje, Y. Chen, R. Singh, A. K. Mourya, W. Zhang, and J. Liu, “Comparative evaluation of pretreatment methods for enhanced anaerobic digestion and biogas production from water hyacinth biomass,” Energy, vol. 119, pp. 229– 237, 2017.

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DOI: 10.14416/j.asep.2024.02.001

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