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Enhancement of β-Cryptoxanthin Production in Three Different Green Microalgae Species Using an Innovative Red LED Wavelength Shift Approach

Sirawit Chuechomsuk, Benjawan Thumthanaruk, Savitri Vatanyoopaisarn, Vilai Rungsardthong, Watcharee Kunyalung, Sonia Mohamadnia, Irini Angelidaki

Abstract


β-Cryptoxanthin is a natural carotenoid pigment with several important functions for human health, including antioxidant, provitamin A, and anticancer activities. Microalgae could be a potential source to produce β-cryptoxanthin instead of its production from plants. In this research study, the effect of the red light-emitting diode (LED) at wavelengths 620 to 750 nm and different intensities of 50, 100, 200, and 300 µmol/m2.s was evaluated for the second phase of the two-stage microalgae cultivation with three microalgae species: Scenedesmus obliquus, Coelastrum morus, and Chlorococcum sp. The results were focused on biomass production, β-cryptoxanthin, and total carotenoid contents to select the microalgae strain that could produce a high amount of β-cryptoxanthin under optimized light conditions. Red LED with an intensity of 200 µmol/m2.s resulted in the biomass production of 5.23 ± 0.27 g/L, 5.72 ± 0.25 g/L, and 5.70 ± 0.17 g/L in three microalgae species of Scenedesmus obliquus, Coelastrum morus, and Chlorococcum sp., respectively. In addition, when compared with 50 µmol/m2.s red LED, the highest β-cryptoxanthin content was obtained from the condition of the red LED at 100 µmol/m2.s for S. obliquus (229.22 ± 5.11 µg/g DCW) and 200 µmol/m2.s for C. morus (311.01 ± 4.75 µg/g DCW) and Chlorococcum sp. (383.68 ± 6.63 µg/g DCW). The applied approach to the proper manipulation of LED color, wavelengths, and intensities in this study will enable the improvement of biomass and enhance β-cryptoxanthin production in three tested microalgae species.

Keywords



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DOI: 10.14416/j.asep.2025.03.001

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