Global Research Trends in Citrus aurantiifolia (1987–2025): Insight into Bioactive Compounds, Biological Activities, and Food Applications from a Bibliometric Analysis

Einstivina Nuryandani, Yusuf Andriana, Tia Erfianti, Muhammad Fatih Abdurrahman, Jasmadi Jasmadi, Indyaswan Tegar Suryaningtyas, Erni Apriyati, Siti Dewi Indrasari, Rofiq Sunaryanto, Donowati Tjokrokusumo, Dedy Kurnianto, Jerry Wungkana, Suzanne Laura Liwu, Christina Litaay

Abstract

Over the period 1987–2025, research on Citrus aurantiifolia (key lime) has expanded substantially due to its rich phytochemical composition, diverse biological activities, and broad application potentials. However, a comprehensive assessment of research trends, collaborations, and thematic evolution of C. aurantiifolia remains limited. This bibliometric study addresses this gap by analyzing 454 Scopus-indexed publications published between 1987 and 2025, providing performance analysis and science mapping with a particular focus on bioactive compounds, biological activities, and food applications. The results revealed a considerable increase in publications since 2010, driven by growing scientific and industrial interest. Research productivity was led by Indonesia, Iran and India, while the highest citation impact was attributed to the United States, which also plays a central role in international collaborations. Keyword and thematic analyses revealed that antioxidant activity, antibacterial activity, essential oils, flavonoids, and phenolic compounds remain among the most prominent research themes. Science mapping showed a strong interconnected landscape among phytochemical characterization, bioactivity evaluation, and application-oriented research, while GC–MS and LC–MS emerged as widely used analytical tools. The thematic evolution analysis demonstrated progression from phytochemical characterization and analytical methodologies toward bioactivity assessment and more recent application-driven topics. Emerging themes such as green synthesis and nano-formulations further indicate increasing diversification toward sustainable and interdisciplinary research. This study provides a comprehensive synthesis of the intellectual structure of C. aurantiifolia research and identifies future directions, including standardized profiling, mechanistic investigations, clinical validations, and scalable nutraceutical applications.

Keywords

References

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DOI: 10.14416/j.asep.2026.08.008

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